Chromosome folding dynamics during stem cell differentiation in bovine

Topic description

This PhD project aims to better understand the three-dimensional organization of animal genomes, and more specifically the dynamics and formation of nuclear compartments.

DNA, which carries genetic information, is folded within the cell nucleus. The genome is organized into two main nuclear compartments that are linked to gene expression and therefore potentially involved in the establishment of phenotypes. However, the causal relationship between nuclear compartments and gene expression remains unclear, and the molecular mechanisms that form and maintain nuclear compartments have not yet been elucidated.

In the first aim, we hypothesize that differences in nuclear compartmentalization between individuals during development play a role in the establishment of phenotypes. The variability of genome organization in wild-type bovine embryonic stem cells and those carrying mutations associated with a specific phenotype, the po...